Muhammad Hayat
Bioactive compounds like enzymes and antioxidants have been added to the feed to ensure its long-term viability and increase its nutritional value. However, feed additives may lose their efficacy and activity during the pelleting and storage processes. The stability of functional activity in feeds enriched with antioxidants, cellulases, and other bioactive extracts was the primary focus of this study. This bioactive extract (FBE) was produced by Aspergillus ibericus through solidstate fermentation of byproducts from olive mills and wineries. With or without lyophilized FBE (0.26 percent w/w) as a supplement, two isolipidic and isoproteic diets were developed. Both diets were kept at room temperature (RT), or 4 °C, for four months. The results indicated that feed stored at 4 °C was more stable for cellulase than for xylanase. The half-lives of cellulase and xylanase were approximately 60 and 14% longer when stored at 4 °C compared to RT, respectively. Antioxidant activity lost in the diet without supplements was the same at both storage temperatures, but antioxidant activity lost less with dietary FBE supplementation when stored at 4 °C. The addition of FBE reduced lipid peroxidation by 17.5% and 19.5%, respectively, when stored at 4 °C or RT. The current study is a first step toward enhancing bioactive compound-based diet storage conditions.
KeywordsFunctional Aquafeeds; Solid-State Fermentation; Antioxidants
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