English Spanish Chinese Russian French Japanese Portuguese Hindi Telugu Tamil

Archives of Clinical Microbiology

  • ISSN: 1989-8436
  • H-Index der Zeitschrift: 22
  • Zitierbewertung der Zeitschrift: 7.55
  • Journal-Impact-Faktor: 6.38
Indiziert in
  • Öffnen Sie das J-Tor
  • Genamics JournalSeek
  • Der Global Impact Factor (GIF)
  • Open-Archive-Initiative
  • Nationale Wissensinfrastruktur Chinas (CNKI)
  • Verzeichnis der Indexierung von Forschungszeitschriften (DRJI)
  • OCLC – WorldCat
  • Proquest-Vorladungen
  • Publons
  • MIAR
  • Kommission für Universitätsstipendien
  • Genfer Stiftung für medizinische Ausbildung und Forschung
  • Google Scholar
  • Scimago Journal-Ranking
  • Geheime Suchmaschinenlabore
  • ResearchGate
Mehr sehen
Teile diese Seite

Abstrakt

Evaluation of Short-Time Method with Tetrazolium Salt and Electron Carrier for Measuring Minimal Inhibitory Concentration of Antimicrobial Agents

Yoshida M, Horino T, Nakazawa Y, Yoshikawa K, Hori S

Background: Minimal inhibitory concentration (MIC) measurements are usually conducted using the agar plate dilution test or the microdilution test. Methods for rapid evaluation of antimicrobial susceptibility using ATP measurements with luciferin-luciferase reagents and a tetrazolium salt have been reported.

Material and methods: We recently measured the minimal inhibitory concentrations (MICs) of various antimicrobial agents against 96 strains of Escherichia coli (1 standard strain and 95 clinically isolated strains) with Cell Counting Kit-8 containing a tetrazolium salt and an electron carrier using the Dry Plate Eiken for MIC measurement and compared the results with that obtained using the standard method.

Results: Agreement exceeded 90% for ampicillin, cefotiam, ceftazidime, flomoxef, aztreonam, imipenem, meropenem, gentamicin, and levofloxacin, and complete agreement exceeded 90% for cefotiam, ceftazidime, flomoxef, aztreonam, imipenem, meropenem, and levofloxacin. Agreement was in the range of 78.1- 86.5% for piperacillin, cefazolin, cefpodoxime, amikacin, and minocycline with complete agreement in the range 55.2-78.1%. Cefaclor had the lowest agreement and complete agreement (42.7% and 38.5, respectively). For the antimicrobial agents with low agreement (piperacillin, cefaclor, cefazolin, amikacin and minocycline), MICs measured by the short-time method were often below onehalf or one-fourth of that measured by the standard method, and regrowth of the bacteria appeared to occur 6 hours after the start of the short-time method.

Conclusion: The results of this study indicate that measuring absorbance with Cell Counting Kit-8 enables counting of viable bacteria. The use of this method for measuring MICs (short-time method) allowed the results of the susceptibility testing to be obtained in 6 hours, and the results correlated well with the results obtained using the standard method. Therefore, this method appears useful for the early detection of drug-resistant bacteria.