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Molekulare Enzymologie und Wirkstofftargets

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A short note on Investigation of DNA-protein Sequence-Specific Interactions with a ds-DNA Array

Zuhong Lu

The use of double-stranded DNA arrays (ds-DNA arrays) for studying sequence specific recognition between DNAs and proteins is a promising method. Here we report the use of a ds-DNA probe with multi operation sites of restriction proteins in the middle sequence to investigate DNA-protein sequence-specific interactions including methylation. We arranged EcoR I site and RNA I site on the same duplex DNA probe to fabricate ds-DNA arrays. We used the ds-DNA arrays to study DNArestriction enzyme reactions before and after duplex DNA methylation under different probe concentration and reaction time conditions. Our results indicated that the ds-DNA arrays can be further biochemically modified and made accessible for interactions between DNAs and proteins in complex multi-step gene-regulation processes.

Keywords

ds-DNA array; Restriction enzymes; Methylase; DNA-protein interactions.